@Zatenm a couple of things:
- Does the "weird high-frequency buzzing sound" depend on the level of the input? In other words, if you turn down the Volume knob on your bass (so your bass isn't hitting the tubes as hard) does it change the weird buzzing sound? Or is the weird buzzing sound always constant, no matter how quiet or loud you play?
- Have you tried using a "cab sim" effect? Very few bassists like the sound of tube overdrive directly into headphones. Most bassists who use tube overdrive as part of their sound seem to end up using a cab sim. (Or--shocking suggestion!--mic the cabinet.)
Finally, an observation based on reading the manuals of your two devices. Your Ampeg has a 60dB signal to noise ratio, whereas your Focusrite boasts an astounding -128dB noise EIN! Your Focusrite is much more "hi fi" than your Ampeg, which means there will be many situations where the Focusrite is "exposing" the old-school gnarliness of the Ampeg. If you want a totally clean and hi fi signal, my advice is to just plug your bass into the Focusrite (bypassing the Ampeg entirely). I personally never ever use my Ampeg for recording (only for jamming with my friends) because the specs are so much better on my audio interface.
PS The reason why you can't "dial it out with EQ even when I completely kill the treble" is because the tube overdrive is happening
after the treble control, in the amp's power stage. Try adjusting the treble EQ in your DAW, I think you will hear, it makes a huge difference
where you put the EQ in the signal chain (before, or after, the overdrive).