What song have you played the most in your life?

Probably Brown Eyed Girl.
I've never played that song. I've been playing 48 years and have never been in a band where anyone suggested learning it. I may be the only bass player in America that hasn't!

I've played in a couple of bands that didn't play Mustang Sally, but I think I've only been in one band that didn't play Honky Tonk Woman, so I'm going to say Honky Tonk Woman is the song I've played the most.
 
Just wanted to chime in to state that I have been in cover bands my entire life and never once played Mustang Sally.

Although I haven’t had to play it in years, most of my bands have always done some variation of What’s Up by 4 Non Blondes. And it was usually always the last song. And it was always miserably boring.